高效液相色谱荧光检测法测定人血浆中帕珠沙星浓度

Determination of pazufloxacin in human plasma by RP-HPLC with fluorimetric detection

目的：建立高效液相色谱法测定人血浆中帕珠沙星浓度。方法：色谱条件：Lichospher C18柱，以甲醇-0．5％三氟醋酸（40：60，v／v）为流动相，激发波长335nm，发射波长450nm。用10％高氯酸沉淀血浆蛋白，离心后取上清液进样。结果：血药浓度测定的线性范围为0．024～19．58mg·L^-1（r=0．9997）。最低检测限为30pg。绝对回收率在84．82％～92．69％．相对回收率在100．7％～104．8％。日内、日间RSD均小于10％。结论：本方法准确可靠、操作简便，适用于帕珠沙星药动学研究。

OBJECTIVE To establish a reverse phase HPLC method for the determination of pazufloxacin in human plasma. METHODS Plasma was deproteinized with 10% perchloric acid. After centrifugation, the supernatant was injected and eluted with methanol- 0. 5% trifluoroacetic acid （40：60,v/v） in a Lichospher C18 column. The wavelengths were 335（ex） and 450 nm （em）, respectively. RESULTS The calibration curve was linear in human plasma within the range of 0. 024 - 19. 58 mg·L^-1 （r = 0. 999 7）. The lowest limit of detection was 30 pg. The average recoveries of pazufloxacin were 100. 7 % - 104. 8%. The RSD of the within-day and between-day were all lower than 10%. CONCLUSION The present study provides a simple and reliable quantitative method for the pharmacokinetic study of pazufloxacin.