香菇子实体多糖分离纯化的研究

STUDIES ON TECHNOLOGY OF PURIFICATION OF LENTINAN

将香菇子实体干粉经热水浸提、乙醇沉淀,得到香菇多糖粗品。香菇多糖粗品经Sevage法去除游离蛋白,H2O2脱色,用DEAE-纤维素柱层析,经蒸馏水、0.05mol/LNaCl、0.1mol/LNaCl、0.2mol/LNaCl、0.3mol/LNaCl和0.4mol/LNaCl洗脱,得到6个香菇多糖级分A～F;然后用SephadexG-200柱进一步纯化香菇多糖B级分,得香菇多糖亚级分B-1和B-2;再研究其中的B-1。采用SephdexG-200柱层析和聚丙烯酰胺凝胶电泳法,证实该多糖组分为均一多糖。

Crude lentinan was obtained from fruitbody of lentinus edodes using following methods： extracting polysaceharide with hot water, precipitating with ethyl alcohol. Free proteins and pigment were removed by Sevage method and H202. Six lentinan fractions were got by DEAE-Cellulose column with eluent of water, 0.05 mol/L, 0.1 mol/L, 0.2 mol/L, 0.3 mol/L and 0.4 mol/L NaCl respectively. Letinan-B was further purified on Sephadex G-200 column, two subfraetions of letinan （B-1, B-2）were obtained. And letinan B-1 would be studied, too.Sephadex G-200 column chromatography and polyacrylamide gel eleetrophoresis illustrated that the fraction B-1 was homogenous polysaeeharide.