大肠恶性肿瘤外周血微转移检测的意义

Significance of Detecting Disseminated Tumor cells in Peripheral Blood of Patients with Colorectal Carcinoma

目的研究大肠癌患者外周血CK19mRNA的表达，并探讨其临床意义。方法采用RT-PCR方法检测41例大肠癌患者和8例肠道非恶性病变手术患者的外周血CK19mRNA的表达。结果8例胃肠道非恶性病变手术患者、8例健康志愿者的外周血CK19mRNA无阳性表达；41例大肠癌患者外周血CK19mRNA阳性表达率分别：单次外周血采样CK19mRNA阳性表达率为41．5％；两次采样总阳性率大肠癌患者为65．9％，外周血重复采样阳性率高于单次采样（P〈0．01）。DukeC、D期患者CK19mRNA阳性表达率高于Duke B期（P〈0．05）。结论大肠癌患者外周血CK19mRNA是一种敏感、特异的检测方法，可以作为检测大肠癌微转移的指标并指导其治疗和预后。

Objective To evaluate the clinical significance of CK19mRNA expression in detecting disseminated tumor cells in peripheral blood ofcolorectal cancer patients.Methods Expression of CK19 mRNA was investigated by RT-PCR in periphral blood in colorectal cancer patients and 8 healthy volunteers and 8 patients with benign gastrointestinal disease.Results There was no positive expression of CK19 mRNA in 8 healthy volunteers and 8 patients with benign gastrointestinal disease.The positive rates of CK19 mRNA in periphral blood with colorectal cancer patients were 41.5% by one single test,and were 65.9 % by two tests.The overall positive rate of CK19 mRNA in periphral blood was higher in two tests than in one single test （P〈 0.01 ）, The positive rates of CK19 mRNA in periphral blood（stage C,D） was higher than the blood（stage B）（P〈0.05）.Conclusion Detection of cancer cells RT-PCR for CK19 mRNA in peripheral blood is sensitive and specific,which possesses clinical signficance in assessing the prognosis and scheme of therapy.