薄层扫描法测定血中甘氨酸茶碱钠片浓度

TLCSDeterminationofglycinetheophylinesodiuminplasma

本文采用薄层扫描法测定血中甘非林浓度实验采用硅胶ＧＦ２５４薄层板，以正丁醇－丙酮－氯仿－浓氨溶液（４∶３∶３∶１）为流动相。在ＣＳ－９３０薄层扫描仪以反射锯齿扫描方式进行测定，λＳ＝２７０ｎｍ，λＲ＝２４０ｎｍ。本法平均回收率为８９．４％ＲＳＤ＝３．１％。茶碱的最小检出量为４２ｎｇ，日内ＲＳＤ为３．９％～２．８％，日间ＲＳＤ为４．４％～３．５％。

TLCSmethodwasestablishedfordeterminationofglycinetheophylinesodiuminplasma.TheTLCplatewascoatedwithsilicagelGF254,usingbutanol-acetone-chloroform-concentratedammoniasolution(4∶3∶3∶1)asdevelopingsolvent.Theplatewasscanedat270nm(λS),240nm(λR)respectivelyinzigzagscanmodewithCS-930scanner(Japan).Theaveragerecoverywas89.4%RSD=3.1%.Theminimumdetectablequantityoftheo-phylinewas42ng.TheRSDwithinadaywere3.9%～2.8%andtheRSDbetweendayswere4.4%～3.5%.