芩柏软膏中黄芩提取工艺的考察

Studies on Extraction Process of Radix Scutellaria in Qinbai Ointment

目的通过提取工艺的研究,寻求芩柏软膏中黄芩的最佳提取条件。方法以黄芩苷为指标,HPLC法为含量测定方法,采用正交设计法,优化黄芩的提取工艺。结果乙醇浓度的影响具有极显著差异(P<0.01),乙醇用量、提取次数及时间的影响具有显著差异(P<0.05)。结论芩柏软膏中黄芩提取工艺为:黄芩饮片,60%乙醇提取3次,时间分别为1、1、0.5h,每次溶剂量为药材重量的10倍。

Objective To seek optimum extraction conditions of Radix Scutellaria in Qinbai ointment by studying on extraction process. Method The optimum extraction regard baicalin as the index. It was investigated by the orthogonal design and determined by HPLC. Result The effect of alcohol concentration has extremely remarkable difference （P〈0.01）. The influence of alcohol consumption, draw number of times and time have remarkable difference （P〈0.05）. Conclusion The optimum extraction process of Radix ScuteUaria in Qinbai ointment obtained was that sliced medicinal herbs of Radix ScuteUaria was extracted with 60% alcohol for 3 times, with the solvent volume 10 times amount each time and extraction time 1, 1, 0.5 h in turn.