欧李茎尖分生组织培养与快速繁殖技术研究

Technology System for Stem-apex Meristem Culture and Rapid Propagation of Prunus humilis

以欧李茎尖分生组织为外植体,研究了在培养基中添加不同的激素成分及不同浓度对不定芽诱导、继代增殖和生根培养的影响。结果表明:不定芽诱导的最佳培养基是MS+6-BA0.4mg/L+IAA0.5mg/L;增殖培养的最适培养基为MS+6-BA0.3mg/L+IAA0.4mg/L;最佳生根培养基是2/3MS+IAA0.5mg/L。应用症状诊断和指示植物鉴定法进行病毒检测,平均脱毒率为73%。

The influences of the factors such as composition and concentration of hormones in culture medium upon adventitious bud induction, shoot proliferation and rooting were studied with the stemapex meristem of Prunus humilis as explant. The results showed that the optimum medium for adventitious bud regeneration was MS＋ 6 - BA 0.4 mg/L. ＋ IAA 0.5 mg/L; the best culture medium for shoot proliferation was MS ＋ 6 - BA 0.3 mg/L. ＋ IAA 0.4 mg/L.; the proper culture medium of rooting was 2/3MS＋ IAA 0.5 mg/L.. And the average virus-free rate was 73% based on the semiological diagnosis and plant indicator identification.