以生物制氢废液筛选产絮菌及产絮条件研究

Screening of Flocculant-producing Bacteria by the Effluent of Biohydrogen Production Reactors and Its Flocculant-producing Conditions

生物絮凝剂因具有安全无毒、对环境无二次污染等优良特性而日益受到人们的重视,但生产成本过高限制了它的工业化应用。为此,开展了利用生物制氢废液筛选高效产絮菌的研究。经分离筛选得到有较强产絮能力的菌6株:BF-1、BF-2、BF-4、BF-6、BF-7、BF-9,这6株菌利用生物制氢废液为底物所产的生物絮凝剂均对高岭土悬浊液的絮凝率>65%。以各株产絮菌的个体和菌落形态特征及生理、生化指标为依据,结合16S rRNA分析确定了这些产絮菌的菌属;通过静态试验,确定了产絮菌的最佳生长和产絮条件。这一技术的开发,使生物制氢工艺可实现全程清洁生产,也为废水的资源化利用提供了新的思路。

Although bio-flocculants have obtained increasing attention for their security, innocuity and no secondary pollution to the environment, the high costs has restricted their industrial use. Screening high-efficiency flocculant-producing bacteria by the effluent of bio-hydrogen production reactors was investigated. Six strains with high flocculant-producing capacity （higher than 65% of flocculating rate）, were isolated. They are named BF-1, BF-2, BF-4, BF-6, BF-7 and BF-9. Through observing the morphological features of individual and colonial bacteria, analyzing parameters of the physiology and biochemistry experiment and combining the results of the 16S rRNA technique, the flocculant-producing bacteria were identified. The conditions of growing and bio-flocculant production were optimized by many batch experiments. This technology allows the process of bio-hydrogen production to become entirely clean, and provides a new method where the effluent is a resource.