总T_3快速检测微流控芯片的制作及应用研究

Rapid analysis of total T_3 on microfluidic chip

目的 建立一种可以用于免疫分析的微流控芯片系统。方法 采用激光直接加工法制备微流控芯片，建立由流体控制系统、免疫反应系统和信号处理系统构成的芯片系统，用免疫竞争法测定总T3。结果 芯片系统可以在20min内完成总T3的分析，所需要的标本量和试剂量为5μl。线性范围为1～10ng／ml，批内CV平均为7．2％。批间CV平均为10．7％，回收率为108．5％。结论 微流控芯片系统用于检测总T3具有快速、灵敏、稳定、可重复使用的特点。

Objective To establish a microfluidic chip system for immunoassay. Methods The direct -write laser micromachining technology was used to fabricate microflidic chip, the whole system was constructed by fluid control system, immunoreaction system and signal analysis system. Then this system was used to detect total T3. Results The analysis was completed within 20min.5μl sample and reagent was needed, there was good linear correlation in the range of 1- 10ng/ml. Average intra-assay and inter-assay precision was 7.2% and 10.7% respectively. Average recovery rate was 108.5Y0. Conclusion The microfluidic chip system is a time-saving, cost-low and convenient method for total T3 analysis.