病毒性肝炎患者内毒素血症及外周血单核细胞Toll样受体4基因表达的研究

A preliminary study of endotoxemia and expressive change of Toll-like receptor 4 mRNA of peripheral blood monocytes in patients of viral hepatitis

目的研究病毒性肝炎患者内毒素血症及外周血单核细胞内Toll样受体4(TLR4)mRNA的表达。方法采用鲎试剂法测定86例肝炎患者和11例对照组的血浆脂多糖(LPS)浓度,外周血单核细胞TLR4mRNA的表达用RT-PCR法检测。结果肝炎患者血浆LPS浓度均有不同程度的升高,高于对照组(P<0.05),重型肝炎组升高最显著,与其他各型肝炎比较差异有显著性(P<0.05);重型肝炎组单核细胞TLR4mRNA的表达升高最明显,与其他各型肝炎及对照组比较差异有显著性(P<0.05),其他各型肝炎与对照组比较差异不明显(P>0.05)。结论各型肝炎均存在内毒素血症,以重型肝炎最为显著,且重型肝炎患者外周血单核细胞内TLR4mRNA的表达水平最高,可能与肝细胞损伤相关。

Objective To study the change of TLR4 mRNA expression of peripheral blood monocytes and endotoxemia in patients of viral hepatitis. Method Peripheral venous blood was obtained from 86 hepatitis patientsand 11 health volunteers served as control. The LPS concentration of plasma was detected by limulus lysate test. RT-PCR was used to detect the expressive change of TLR4 mRNA. Results In the patients, LPS concentration of plasma was elevated in different degrees compared with control group （ P 〈 0.05）. In severe hepatitis group, the elevation of the LPS concentration was higher than that of other hepatitis group （ P 〈 0.05 ）. The expression of the TLR4 mRNA in severe group was increased significantly compared with those of control group （P 〈 0, 05）, However,in other hepatitis group ,TLR4 mtLNA expression was not significantly changed compared with those of control group （ P 〉 0. 05）. Conclusion Endotoxemia was present in various hepatitis group, which was more prominent in severe hepatitis group. The expression of TLR4 mRNA in peripheral blood monocytes in severe hepatitis patients might be closely correlated with liver injury.