控制微流控芯片电泳中的蛋白质吸附

Control of protein adsorption in microfluidic chip electrophoresis

目的探讨抑制微流控芯片电泳中蛋白质的吸附,以提高芯片电泳蛋白质的分离效率及重现性。方法以蛋白质标准品、临床尿液标本为实验对象,通过提高缓冲液的pH值、在缓冲液中加入适当的添加剂等方法,进行芯片蛋白电泳,并与常规琼脂糖电泳结果进行比较。结果使用pH值10.5硼酸缓冲液,转铁蛋白连续进样出峰时间的变异系数(CV)为5.22%;在pH值10.5硼酸缓冲液中加入1%的乙胺,能提高芯片电泳检测尿液蛋白的分离度、重现性,电泳结果与REP全自动电泳仪结果一致。结论使用该方法可有效地抑制芯片泳道壁对蛋白质的吸附,分离效率大为提高。

Objective To establish a method of controlling protein adsorption in microfluidic chip electrophoresis to enhance efficiency and reproducibility of protein electrophoresis. Methods The standard model proteins and clinical u rine specimens were analyzed by improvement of pH and introduction of additive and compared with gelose electrophoresis. Results With the pH 10.5 borate buffer, moving time coefficient of variation （CV） of transferrin was 5.22%. Introduction of 1% ethylamine as additive could improve separation efficiency and reproducibility of urinary protein electrophoresis which was consistent with results of REP. Conclusions Protein adsorption to channel wall could be restrained effectively and separation efficiency could be improved with the method.