摘要
目的利用在体动物模型观察镀铜织物物理金属网电磁屏蔽布(EM-4)对电磁脉冲(EMP)辐照后免疫组织的防护效果,并在离体Jurkat细胞损伤模型上探讨药物防治的可能性。方法选定Wistar大鼠和Jurkat细胞进行6×104V/mEMP辐照,复制动物模型和细胞模型,应用血生化分析、光镜、噻唑蓝(MTT)、流式细胞术检测研究EM-4屏蔽布和药物4,5,7三羟异黄酮(Cenistein)对EMP辐照后免疫组织和细胞的防护效果。结果(1)EM-4屏蔽布防护组大鼠外周血白细胞和淋巴细胞计数在照后恢复快于无防护组。(2)Genistein能部分阻断EMP辐射对Jurkat细胞增殖的抑制和S期细胞的减少。结论EM-4和蛋白酪氨激酶(PTK)抑制剂Genistein对6×104V/mEMP所致细胞免疫功能损伤、细胞增殖活力下降及离体细胞DNA合成受阻有一定防护效果。
Objective To study the protection effect of metal net- like defense cloth in vivo and genlstein on Jurkat cell after electromanetic pulse（EMP）. Methods Wistar rats and Jurkat cells were exposed to high power EMP of 6 - 10^4 V/m as experimental models. By various methods such as biochemical tests, light microscopy, methyl thiazolyl tetrazolium （MTT）, flow cutometry, the protection effect of metal net-like defense cloth and protein tyrosine kinase（PTK）inhibitor Genistein on immune organization and cell were investigsted. Results （1）Peripheral blood lymphocyte of rats sheltered with metal net- like defense cloth （EM - 4） recovered, more quickly than that of rats unsheltered; （2）Pretreatment with gensitein could partially block the degrassion of EMP on the proliferation activity and Jirkat cell in S phsase instantly. Conclusion （EM - 4） and PTK inhibitor Genistein are effective to protect the immune function, cell proliferation activity and DNA synthesis from EMP radiation.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2006年第9期1068-1069,共2页
Chinese Journal of Public Health
