挥发性有机化合物甲醛、苯联合细胞毒性作用

Eytotoxicity of volatile organic compounds on Chinese hamster lung cells

目的研究2种主要的挥发性有机化合物(VOCs)甲醛、苯对中国仓鼠肺(CHL)细胞的联合细胞毒性作用。方法将CHL细胞暴露于不同浓度的甲醛、苯及两者的混合溶液中。采用四甲基偶氮噻唑蓝(MTT)法检测甲醛和苯对CHL细胞相对增殖率的影响,评价其联合细胞毒性作用。结果作用48 h后,苯对CHL的IC50为48.35μg/ml,甲醛处理组的IC50为75.10μg/ml。两者联合作用的类型为协同作用。结论甲醛和苯可引起细胞损伤,具有一定的细胞毒性作用,两者混合作用的细胞毒性远远大于两者单独作用的细胞毒性。

Objective To evaluate the cytotoxicity of volatile organic compounds i.e. formaldehyde and benzene on Chinese hamster lung （CHL） cells in vitro. Methods CHL cells were exposed to formaldehyde and benzene at different concetration respectively and simultaneously. Cell growth was tested by methylthiazoletrazolium（MTT） assay and absorbency was determined to calculated the cell relative growth rate. Results According to MTT assay, the IC50（48 h） of benzene for inhibiting CHL cell proliferation was 48.35μg/ml and the IC60（48 h） of formaldehyde was 75.10μg/ml. The results showed that a synergistic effect existed between the two chemicals. Conclusion Formaldehyde and benzene have a direct cytotoxicity on CHL cells. The toxicity exposed to formaldehyde and benzene simultaneously is greater than that of exposed to formaldehyde and benzene respectively.