砷暴露母子砷代谢特点及DNA损伤差异

Study on characteristics of arsenic metabolism and DNA damage between mother and child under arsenic exposure

目的通过尿中各种形态砷水平,探讨高砷暴露下儿童砷代谢特点;测定尿中8-羟基脱氧鸟苷(8-O-HdG)水平,初步探讨砷暴露对儿童DNA的损伤情况;并比较砷暴露对儿童与成人的不同影响。方法以氢化物发生-冷原子捕获-原子吸收分光光度法测定尿中各种砷化物的含量;以试剂盒法测定尿8-OHdG水平。结果砷暴露组妇女和儿童尿中无机砷(iAs)、二甲基砷(MMA)、甲基砷(DMA)及总砷(tAs)水平均显著高于对照组;砷暴露母子间,子女尿中iAs、DMAt、As及DMA/tAs水平显著高于其母亲,而MMA/(MMA+DMA)显著低于其母亲。母子间8-O-HdG水平差异无统计学意义。结论高砷暴露下,儿童二甲基化能力高于成人,DNA氧化损伤与成人相比不明显。

Objective To explore the characteristics of arsenic metabolism and DNA damage of children under arsenic exposure through the detection of arsenicals and 8-OHdG in urine, and to explore whether there is a difference of arsenic exposed effects between children and adults. Methods Arsenicals were measured by the hydride generation-cold iron captureatomic absorption spectrophotometry; 8-OHdG levels were detected by detection kit. Results The level of inorganic arsenic （iAs）, monomethylarsonate acid（MMA）, dimethylarsinic acid（DMA）and total arsenic（tAs）of female and children of the exposed group were significantly higher than those of control＇s. Between the arsenic exposed mother and child, the level of iAs, DMA, tAs and DMA/tAs of the child were markedly higher than those of their mother＇s, while MMA/（MMA＋ DMA）was significantly lower than that of their mother. There was no significant difference of 8-OHdG level between mother and child. Conclusion The secondary arsenic methylation of children under arsenic exposure is stronger than that of adults. There is no significant difference of DNA damage between arsenic exposed children and adults.