Apoptin基因对小鼠S180荷瘤细胞抑制作用

Inhibition effects of apoptin gene on murine sarcoma 180 in vivo in mice

目的研究肿瘤物异性凋亡基因(apoptin gene)对肿瘤细胞的抑制作用。方法以S-180肉瘤细胞的荷瘤小鼠为模型,用克隆的肿特异性凋亡基因的真核表达载体直接进行基因抑制,分别测定小鼠体内的肿留大小、荷瘤小鼠生存期和肿瘤细胞的凋亡。结果与对照组相比,基因抑制组小鼠一般生长状态良好、体内肿瘤生长明显减缓(P<0.01)、生存期延长、体重增加,在基因抑制的肿瘤组织中可检测到肿瘤特异性凋亡基因mRNA的表达及肿瘤细胞的凋亡。结论肿瘤特异性凋亡基因在体内具有明显的抗肿瘤作用,对开展肿瘤的基因治疗有一定意义。

Objective To study the inhibition effects of apoptin gene on inducing regression of murine sarcoma 180 （S180）and prolonging the survival term of mice in vivo. Methods Mice beating S180 were used as the model and treated with the apoptin gene. The survival term, weight of the tumor and the apoptosis of tumor cells were detected respectively. Resuits pcDNAA3 plasmid, which contains apoptin gene, could result in regression or complete remission of S180 grown as xenografts in mice and significantly increased their survival long term by intratumoral transfer. Early after intratumoral injection, apoptin mRNA could be detected in significant quantities by RT - PCR. Furthermore, cell death and disruption of the tumor integrity were apparent in the transduced regions. Conclusion This experimental gene therapeutic strategy constitutes a unique example of specific antitumor activiy using apoptin gene.