猪卵母细胞去核方法的改进

A Modified Enucleation Method of Porcine Oocytes

对猪体细胞克隆技术中关键步骤之一的去核方法作了较深入的研究。结果表明,由本实验室改进的Willadsen去核法———二步挤压去核法,无论在去核操作成功率(73.9%)上还是在去核效率(81.2%)上都明显好于McGrath-Solter去核法(42.5%、67.4%,P<0.05);同时,本试验还发现,卵母细胞成熟培养36 h后去核组去核效率(89.1%)显著地高于成熟培养44 h后去核组(55.8%,P<0.05),而核移植重构胚的发育率2个组间无显著差异。

This experiment was conducted to study enucleated method, which is one of the key techniques for animal cloning. The results show that the modified Willadsen＇s method for enucleation ,two-step-squeezing method ,is significantly better than McGrath-Solter＇s method in the enucleated rates （73.9%,42.5%,P〈0.05） and the rates （81.2%,67.4%,P〈0.05） of absolutely removing nuclei matter. It was also found that the rates（89. 1%） of absolutely removing nuclei matter for 36 hmatured-in-vitro oocytes is significantly higher than that for 44 h（55.8% ,P〈0.05）.