HPLC-ELSD测定三七通脉片中三七皂苷R_1、人参皂苷Rg_1及Rb_1的含量

Determination of Notoginsenoside R1,Ginsenoside R1 and Rb1 in Sanqi Tongmai Tablet by HPLC-ELSD

目的:用HPLC—FLSD测定三七通脉片中三七皂苷R1、人参皂苷Rg1及Rb1的含量。方法:色谱柱填料为十八烷基硅烷键合硅胶,流动相为乙腈-水,梯度洗脱,漂移管温度105℃,载气流速3．0L·min-1。结果:三七皂苷R1、人参皂苷Rg1及Rb1分别在0．300-3．000μg,1．01-10．100μg,0．976-9．760μg之间呈良好的线性关系;制剂中3种成分的平均回收率分别为96．5％(RSD 1．2％),98．3％(RSD 1．5％),97．6％(RSD 2．1％)。结论:该方法简便、准确、分离效果好,无干扰,可用于三七通脉片的质量评价。

Objective： To establish the quantitative method of notoginsenoside R1, ginsenoside Rgl and Rbl in Sanqi Tongmai Tablet by HPLC - ELSD. Methods： The column was packed with 5μm Diamonsil C（18） stationary phase. The mobile phase consisted of acetonitrile - water, eluted in gradient mode. The temperature of drift tube was 105℃ and the nebulizer nitrog flow rate was 3.0L min^-1. Results：The linear ranges of the three components were 0. 300-3. 000μg, 1. 010- 10. 100μg, 0. 976- 9. 760μg. Conclusion： The method is simple, accurate with better seperated effect. It can be nsed for quality evaluation of Sanqi Tongmai Tablet.