摘要
目的观察羊膜对体外培养成纤维细胞的增殖及TGF-1β分泌含量的影响。方法体外培养的成纤维细胞随机分为3组,2组分别与羊膜、塑料片共同培养,1组设为空白对照。培养后通过细胞计数,流式细胞术及TGF-1βEL ISA试剂盒进行检测。结果(1)细胞计数检测:3组均表现出有限细胞生长过程。细胞群体倍增时间羊膜组为68.5 h,塑料片组为62.5 h,空白对照组为64 h。(2)流式细胞仪检测:羊膜组S期细胞数量比空白对照组少5.1%,比塑料片组少8.7%。(3)EL ISA检测TGF-1β含量:培养24 h细胞TGF-1β含量羊膜组为95.625 pg/m l,空白对照组为123.25 pg/m l,塑料片组为500.25 pg/m l,48 h细胞TGF-1β含量羊膜组为166.825 pg/m l,空白对照组为478.125 pg/m l,塑料片组为1 020 pg/m l。结论(1)羊膜对体外培养成纤维细胞的增殖有抑制作用。(2)羊膜对体外培养的成纤维细胞TGF-1β分泌含量有显著下调作用。(3)羊膜可能为一种较理想的防止椎管内及肌腱等术后黏连的移植材料。
Objective To observe the effects of amniotic membrane on the proliferation and the content of TGF-β1 in cultured human fibroblasts. Methods The cultured human fibroblasts were divided into three groups at random and cultured with amniotic membrane（Am）,plastic（P） and sham（S） respectively followed by means of cell-count,flow eytomery and ELISA kits to find the effects of amniotic membrane on the cultured human fibroblasts. Results （1）Cell count ：all of the three groups showed the growth of finite cell line. Population double time of cell cultured with amnlotic membrane is 68.5hr,wlth sham is 64hr,with plastic is 62.5hr. （2）Flow cytomery：the proportion of cell cultured with amniotic membrane in S-period is 5. 1% less than cell cultured with sham,and 8. 7% less than cell cultured with plastic, （3）ELISA kits：TGF-β1 content of cell cultured with amniotic membrane in 24hr is 95. 625 pg/ml ,with sham is 123.25 pg/ml ,and with plastic is 500.25 pg/ml ;TGF-β1 content of cell cultured with amniotic membrane in 48hr is 166. 825 pg/ml,with sham is 478. 125 pg/ml,with plastic is 1 020 pg/ml. Conclusion （1） Amniotic membrane can prevent the proliferation of cultured human fibroblasts. （2）Amniotic memhrane can decrease the content of TGF-β1 of cultured human fibroblasts. （3）Amniotic membrane may be a kind of proper transplantation material against the adhesion of spinal canal and tendon after operation.
出处
《淮海医药》
CAS
2006年第5期353-355,共3页
Journal of Huaihai Medicine
