测定植物提取液中生物碱含量的间接萃取比色法研究

A Research of Indirect Extraction-Spectroophotometric Mathod for Determinating the Content of Alkaloid in the Extract of Plants

本文报告了在非离子表面活性剂存在下，用溴甲酚绿离子缔合萃取光度法测定植物提取液中生物碱的总量．比较了五种提取方法，探讨了测定的优化条件，确定用水饱和的正丁醇溶液提取效果最佳．测得一些植物的生物碱含量约０．１－０．９％．加标回收率９２％，相对标准偏差７％，结果令人满意．

In this paper,it is reported that with the existence of nonionic surfactants the total amount of alkaloid in the extract of plants is determinedby extraction-spectrophotometric method of bromocresol green ion-association.Five extracting methods are compared to explore the optimum condition,and the water-saturated n-butanol is warranted to be the best extracting agent.The content of alkaloid in the extract of several plants is about 0.1￣0.9%,the rate of recovery of the added satndard liquid is over 92%,relative standard deviation is about 7% (n=5),The results are satisfactory.