摘要
建立了检测促卵泡素(FSH)抗体的间接ELISA方法,用该法检测由FSH-BSA免疫BaLb/c小鼠所分离的5份待检血清,结果均为阳性。通过反复试验,确立间接ELISA检测FSH抗体的最佳反应条件,即酶标二抗工作浓度为1∶1 200,抗原包被浓度10μg/mL,待测血清最佳稀释度为1∶500,封闭液为1.0%的脱脂奶粉,同时设置抗原阻断试验对结果进行验证。
An indirect enzyme-linked imnunosorbent assay（ELISA） was standardized to detect the antibody against follicular-stimulating honnone（FSH）. Being tested with the indirect ELISA, 5 serum samples which were acquired by the FSH-BSA immunized BaLb/cmouses were all positive, The optimum work concentration of the indirect ELISA was established,Polystyrene micro-titer plates were coated by the purified FSH at theconcentration of 10μg/ml, The working concentration of sertan samples was 1：500 and the sheep-against-mice IgG labeled by HRP was 1 ： 1 200,The confining liquid was 1,0% defatted milk powder, Antigen-blockage test was designed to check the results.
出处
《安徽农业科学》
CAS
北大核心
2006年第17期4223-4224,4226,共3页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金资助项目(39770545)
西北农林科技大学青年骨干教师支持计划项目(01140305)
