摘要
为了开发利用油菜籽壳这一油脂工业生产副产品,采用固态发酵方式培养木霉T5,研究以油菜籽壳为主要原料生产饲用纤维素酶最佳工艺条件。结果表明,木霉T5在油菜籽壳／啤酒糟／麸皮=5／2／3,NH4NO3 2％,CaCl2 0．1％,Tween 80 0．1％基质上;加水量为120％,接种量为10％,置30℃下培养60 hr,产酶量可达80．116IU／g,具有较好应用前景。
The optimal condition for feeding-cellulase produced by Trichoderma spT5 solid state ferment rapeseed hull was studied. The result shows that the composed of medium were：rapeseed hull/ brewer＇s grains/wheat bran = 5/2/3;NH4NO3 2% ; CaCl2 0.1% ; Tween80 0.1% ;water 120%. The optimum for producing cellulase were inoculate amount 10%; temperature 30℃ and cultivation for 60 hr. The cellulase production by Trichoderma spT5 can reach 80.116 IU/g.
出处
《粮食与油脂》
2006年第9期21-23,共3页
Cereals & Oils
关键词
油菜籽壳
固态发酵
纤维素酶
木霉
rapeseed hull
solid state fermentation
cellulase
trichoderma sp
