经腹腔诱导免疫耐受抑制EAE的实验研究(英文)

Suppression of Experimental Autoimmune Encephalomyelitis by Inducing Immunologic Tolerance Via Peritoneal

目的通过建立实验性自身免疫性脑脊髓炎(EAE)动物模型,研究经腹腔诱导免疫耐受的方法及机理。方法腹腔内注射可溶性髓鞘碱性蛋白(MBP)或转输可溶性MBP致敏的腹腔抗原提呈细胞(MBP-APC),观察各组动物EAE的发病情况,检测各组动物迟发型超敏反应(DTH)和淋巴细胞体外增殖应答。结果腹腔内注射MBP及MBP-APC的动物发病率明显低于EAE组,临床症状减轻,由MBP引起的DTH和体外特异的MBP淋巴细胞增殖反应也明显降低。结论腹腔注射MBP或MBP-APC可引起粘膜耐受,抑制EAE的发生。

Objective To investigate the method and mechanism of immunologic tolerance induction via peritoneal on experimental autoimmune encephalomyelitis （EAE） Methods The animals were administrated with the soluble myelin basic protein （MBP） or transferred MBP sensitizing antigen presenting cells （MBP-APC） by intraperitoneal （i. p. ）. The clinical symptoms of EAE were observed. The delayed type hypersensitivity （DTH） and proliferative responses to MBP in vitro were tested. Results The incidence of EAE and severity of the disease were decreased in the i.p. injection of MBP group and i.p. injection of MBP-APC group contrast to the EAE group, In addition, DTH responses and the antigen specific proliferative responses to MBP in vitro were markedly suppressed. Conclusions EAE can be inhibited by intraperitoneal administration of MBP or MBP-APC via induction of specific immunologic tolerance.