乳腺癌组织中MMP-2、MMP-9表达及预后意义

Expressions and Prognosis Importance of MMP-2 and MMP-9 in Breast Cancer

目的:探讨基质金属蛋白酶MMP-2、MMP-9在乳腺癌中的表达情况及与临床预后的关系。方法:采用组织芯片及免疫组化S-P法检测了183例乳腺癌组织中MMP-2、MMP-9的表达水平。结果:①乳腺癌组织中MMP-2和MMP-9的阳性表达率分别为77·0%(141/183)和65·6%(120/183)。乳腺癌组与良性对照组MMP-2、MMP-9的表达具有显著性差异(P<0·01)。②MMP-2的表达与肿瘤大小有关(P=0·010),与临床TNM分期、淋巴结转移状态、组织学分级密切相关(P均<0·01);MMP-9的表达与组织学分级有关(P=0·012),与肿瘤大小、临床TNM分期、淋巴结转移状态密切相关(P均<0·01);MMP-2和MMP-9的表达与年龄、月经状态、病理类型以及雌孕激素受体状态无关。③MMP-2、MMP-9高表达组10年生存率均低于低表达组。结论:MMP-2、MMP-9均可作为乳腺癌患者预后判断的指标。

Objective. To observe the expressions of MMP-2 and MMP-9 in breast cancer, and investigate their correlation with the clinical prognosis for breast cancer. Methods： MMP-2 and MMP-9 were detected by Microarray and S-P immunohistochemieal technique in 183 patients with breast cancer. Results. ①The expression levels of MMP-2 （77.0 % ） and MMP-9 （65.6 % ） in the breast cancer tissues were significantly higher than those of benign breast diseases （P〈0. 01）. ②MMP-2 was correlated with the tumor size （P =0. 010）, and significantly correlated with clinical TNM stage, histological grade, and lymph node status. MMP-9 was correlated with the histological grade （P = 0. 012）, and significantly correlated with tumor size, clinical TNM stage, and lymph node status （P 〈0.01）. MMP-2 and MMP-9 had no correlation with age, pathological type, the status of ER and PR. ③The survival rate of 10 years in the group of MMP-2 and MMP-9 over-expression was lower than that of low-expression the group. Conclusion： MMP-2 and MMP-9 all could serve as markers for prognosis judgment of breast cancer patients.