丹参联合胞二磷胆碱治疗大鼠脑挫伤的机理研究

Morphological Change of Brain Contusion Treated by Salvia Miltiorrhiza

目的:探讨丹参与胞二磷胆碱联合应用治疗脑损伤的机制。方法:取成年wistar大鼠,以自由落体法致大鼠脑挫裂伤,采用721分光光度计检测伤后24h、7d和14d时,治疗组与对照组脑组织中SOD、MDA、GSH-PX和Ca2+含量。结果:治疗组SOD和GSH-PX活性提高,而且与模型组及对照组比较,SOD有显著性差异(P<0·05)。治疗组脑组织Ca2+含量明显降低。结论:丹参在治疗脑损伤时,可以提高SOD活性,拮抗自由基和钙离子超载对脑的损伤。

Objective： To investigate the morphological changes of brain contusion treated by Salvia miltiorrhiza, methods：The Feeney＇s falling body injury method was used to make 45 wistar rats into brain contusion model. Then the rats were randomly divided into two groups, i.e. experimental group including 30 animals were treated with Salvia miltiorrhiza and Cytidine diphosphale choline through abdominal injection, 15rats without any treatment were taken as control group. After 2d, 14d, and 28d of brain contusion, the morphological change was observed at the injured area by LM and EM and the contents of SOD, MDA in serum were observed. Results： The morphological observation revealed that the lesioned neuron restored faster and scar tissue formated early in the experimental group. The concentration of SOD in serum was significantly higher in the experimental group than that in the control group （P 〈0.05）, the concentration of MDA was lower in the experimental group than that in the control group. Conclusion： It might promote the restoration of brain contusion to treat the rats with Salvia miltiorrhiza and Cytidine diphosphale choline.