血管内皮生长因子-C、高危型人乳头瘤病毒在子宫颈上皮内瘤样病变及子宫颈鳞癌中的表达和意义

Investigation of the expression of vascular endothelial growth factor-C and high risk human papillomavirus in different stages of human cervical lesions

目的探讨人乳头瘤病毒(HPV)感染和血管内皮生长因子-(CVEGF-C)在子宫颈癌的形成及演进过程中可能的作用机制。方法采用PCR技术检测16例正常子宫颈,65例子宫颈上皮内瘤样病变(CIN),其中,CINⅠ级20例、CINⅡ级24例、CINⅢ级21例,26例子宫颈浸润性癌(子宫颈癌组)组织中高危型HP(VHR-HPV)的感染情况,同时采用免疫组化SABC法检测VEGF-C的表达水平。结果VEGF-C在CIN及子宫颈鳞癌组织中均表达,并且随着病变程度的增强VEGF-C的表达呈渐进性增强,而正常的子宫颈组织中无表达。HR-HPV同CIN及子宫颈癌均显著相关。CIN及子宫颈癌组织中VEGF-C的表达同HR-HPV的感染显著相关。结论VEGF-C蛋白的表达同HR-HPV感染显著相关,可能是子宫颈癌血管形成的早期预测指标。

Objective To study the pathogenetic role of HPV infection and vascular endothelial growth factor-C in the development of cervical carcinomas. Methods HPV16 DNA was tested in cervical intraepithelial neoplasia （CIN） 11Ⅰ, cervical carcinomas and normal cervical tissue by PCR. and the expression of vascular endothelial growth factor-C protein was examined by immunohistochemistry. Results The expression of VEGF-C became stronger and stronger with the progression of cervical diseases from chronical inflammation to CIN and to invasive carcinoma of cervix uteri, but no expression in normal cervical tissue. There was significant correlation between HR-HPV and VEGF-C expression in CIN and cervical carcinomas （odds ratio are 19.12 and 20.49; 95 % confidence interval are 2.31-157.8 and 3.28-226.09）. Conclusions The expression of VEGF-C, maybe a sensitive maker, is closely related to HR-HPV infection.