HPLC测定花锚草及乙肝健片中1-羟基3,4,5-三甲氧基酮的含量

Determination of 1-Hydroxy-3,4,5-methoxy xanthone in Haleniaeplliptica D.Don and Yiganjian Tablets by HPLC

目的:建立HPLC法测定花锚草及乙肝健片中1-羟基3,4,5-三甲氧基口山酮含量的方法。方法:采用HPLC法,使用HypersilC18柱(5μm,4.6mm×250mm),以甲醇-0.5%的磷酸(60∶40)为流动相,流速为1.0ml/min-1,检测波长243nm。结果:1-羟基3,4,5-三甲氧基口山酮在0.01416μg^0.1062μg,(r=0.9999)范围内与峰面积呈良好的线性关系,平均回收率为98.3%,RSD=2.07%(n=6)。结论:本法简便、快速、结果满意,可作为花锚草及乙肝健片质量控制的依据。

Objective To establish a method for determination of 1-Hydroxy-3,4,5-methoxy xanthone in Halenia eplliptiea D. Don and Yiganjian Tablets by HPLC. Methods HPLC was used with Hypersil C18（5 μm,4.6 mm×250 mm）. The mobile phase was composed of methanol -0. 5 %phosphoric acid（60 ： 40,v ： v）. The detection wavelength was at 243nm and the flow rate was 1.0 ml/min-1, Results The linear response range lay in 0. 014 16-1. 062 ptg of 1-Hydroxy- 3,4,5-methoxy xanthone （ r= 0. 999 9, n = 6）. The average recovery was 98.3 %, RSD= 2.07 %. Conclusion The method is simple and satisfactory and suitable for quality control of Halenia eplliptiea D. Don and Yiganjian Tablets.