牡丹矮化品种亲缘关系的AFLP分析

Phylogenetic relationship of dwarf tree peony cultivars by AFLP analysis.

利用荧光标记AFLP技术,采用8对M+3和P+3引物组合对4个牡丹野生种和26个矮化及高大品种间的亲缘关系进行了分析.共获得1 133条可统计的条带,其中948条呈多态性,多态性带百分率达84%,揭示了牡丹组植物丰富的遗传多样性.聚类结果表明:在受试的4个牡丹野生种中,与栽培牡丹亲缘关系从近到远依次为:杨山牡丹、矮牡丹、紫斑牡丹和卵叶牡丹;矮牡丹和卵叶牡丹有较近的亲缘关系.牡丹品种间的聚类结果表明,部分矮化品种、高大品种分别相聚,而一些矮化与高大品种也相聚.不同相似系数的遗传聚类划分与株高间并没有完全一致的关系,但在其他性状相差不大时,株高相近的品种间亲缘关系相对较近.

AFLP technique was used to elucidate the 26 dwarf and tall types of tree peony cultivars systematic relationships among four Chinese wild species and Eight AFLP primer combinations （M ＋ 3 and P ＋ 3） were used to produce 1 133 recognizable bands, among which 948 bands were polymorphie. The percentage of polymorphie bands was 84%, suggesting abundant genetic diversity among Paeonia sect. Moutan. Clustering analysis showed that the systematic relationships among the four wild species and cultivars were in the order of P. ostii, P. qiui, P. rockii and P. jishanensis from close to far. Among these species, the closest relationship was observed between P. fishanensis and P. qiui. The clustering results based on DNA bands among the cultivars showed that some dwarf and tall peony types clustered separately and some of both also clustered each other. Genetic clustering classification of different resemble coefficients was not consisted with that based on the plant height. However, the two clustering results were identical among the eultivars with same plant height and similar phenotypes.