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马立克氏病毒CVI988的TK及gB基因启动子的扩增

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摘要 根据GenBank公布的MDV GA株全基因组序列设计引物,以CVI 988/R ispens感染鸡胚成纤维细胞的基因组总DNA为模板,利用PCR的方法分别扩增了CVI 988/R ispens的TK及gB基因启动子基因,克隆入pGEM-T-easy载体并进行测序,结果表明CVI 988/R ispens的TK及gB基因启动子序列均存在TATA盒和CAAT盒等真核启动子所具有的保守序列,且与MDV GA株的相应基因序列完全一致,为进一步研究该启动子在MDV病毒载体中的表达调控提供了物质条件。
作者 刘红梅 叶建强 秦爱建 祁克宗
机构地区 安徽农业大学动物科技学院 扬州大学动物预防医学重点实验室
出处 《畜牧与兽医》 北大核心 2006年第6期49-51,共3页 Animal Husbandry & Veterinary Medicine
关键词 CVI 988/Rispens TK启动子 gB启动子
分类号 S852.65 [农业科学—基础兽医学]
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参考文献5

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畜牧与兽医
2006年 第6期

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