摘要
以筛选出的具有脱硫能力的施氏假单胞菌UP-2为固定化研究对象,二苯并噻吩(DBT)为生物催化脱硫模型化合物,考察了脱硫菌UP-2的固定化操作条件和固定化细胞使用条件.结果表明,当包埋剂聚乙烯醇(PVA)浓度为10%、添加剂海藻酸钠(SA)浓度为0.2%、液菌比为20:1时,在4℃、含有1%CaCl2的饱和硼酸中交联24h后,可以得到脱硫性能很好的固定化细胞小球;在30℃、pH值为7.0的体系中反应6d,可将浓度为576mg/L的DBT降解70%左右,固定化细胞降解DBT的比活性由未固定化细胞的0.49mmol/gdw增加到6.39mmol/gdw,使用寿命高达800h以上.
The condition of immobilization operation of Pseudomonas stutzeri UP-2 and the condition of application of immobilized cell were inspected, using Pseudomonas stutzeri UP-2 possessing desulfurizing capabilities as the immobilization object, and dibenzothiophene （DBT） as a biocatalytic desulfurization model compound. The immobilized cell small globule of very good desulfurization function could be obtained when the concentration of PVA carder was 10%, concentration of additive SA was 0.2%, the liquid bacteria ratio was 20：1 after 24h crosslinking in saturated solution of boracic acid containing 1% CaCl2 at 4℃. The reaction of 6d in the system of pH value 7.0 of 30℃ could degrade about 70% of DBT of the concentration of 576mg/L. The specific activity of the immobilized degrading DBT increased from 0.49mmol/gdw to 6.39mmol/gdw, with the application life raised reaching above 800h.
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2006年第B07期92-96,共5页
China Environmental Science
基金
中国石油天然气集团公司中青年创新基金资助项目(w000424)
