慢性胃病患者口腔与胃内幽门螺杆菌研究

Study of Helicobacter pyiori in oral cavity and stomach from chronic gastritis patients

目的：研究慢性胃病患者口腔中是否存在幽门螺杆菌(Hp),并分析胃病患者牙周状况与幽门螺杆菌的关系。方法：依据特异的尿素酶C基因和cag A基因设计引物,建立聚合酶链反应(PCR)方法。检测65例慢性胃病患者不同牙位龈上和龈下菌斑中的Hp。结果：65例慢性胃病患者胃黏膜标本中,PCR检测阳性标本58例(89．3%),龈下菌斑Hp阳性率(47．7%),高于龈上菌斑中的Hp阳性率(26．2%)。牙周袋深度(PD)≥4 mm部位菌斑中Hp检出率显著高于PD＜4mm的部位(P＜0．05)。结论：口腔Hp是慢性胃病患者Hp感染的重要来源。

Objective： To investigate the prevalence of Helicobacter pylori（Hp） in the oral cavity of gastritis patients, and analyze the relationship of the patients＇ chronic periodontal disease and Hp. Method： A polymerase chain reaction （PCR） assay with oligonucleotide primers homologous to a portion of the urease C and cytotoxin-associated gene A （cag A） of Hp was used to test for the presense of Hp in dental plaque. 65 gastric disease patients were recruited to detect Hp in supra- and subgingival plaque. Result. 65 chronic gastritis patients, 58 patients （89.3 % ） had at least one Hp positive dental plaque sample. The Hp positive rate was significantly higher in supragingival plaque （47.7 % ） than in subgingival plaque （26.2%）. The prevalence of Hp was positively correlated with probing depth. Conclusion. Oral Hp infection may be an important source of gastric Hp infection.