复发性阿弗它口炎与MHC-DR基因遗传多态性研究

MHC-DRBI gene polymorphism in patients with RAS

目的：探讨复发性阿弗它口炎(RAS)易感的分子免疫遗传学机制和确立一种较为实用的MHC—Ⅱ类基因检测方法。方法：采用聚合酶链式反应和顺序特异性引物(PCR—SSP)基因分析法,对31例复发性阿弗它口炎患者及30例无血缘关系的健康人的人类主要组织相容性复合体(MHC—Ⅱ类)DR各等位基因及亚基因进行检测分析,并将该方法与其它检测MHC—Ⅱ类基因的方法进行对比。结果：结果表明,MHC-DRB1*0301基因与RAS呈正相关(RR=4．696,P＜0．05),其它MHC-DRB1*各等位基因未见异常。结论：MHC-DRB1*0301基因可能是北方汉族人RAS的致病易感基因之一。本文所采用的方法(PCR—SSP法)具有快速、简便、敏感、准确和可靠等优点。

Objective： In order to study the correlativity between MHC-DRBI ＊ gene polymorphism and Recurrent aphthous stomatitis（RAS）. Method： MHC-DRBI ＊ gene polymorphism in 31 patients with RAS and 30 normal control subjects was analyzed by using the polymerease chain reaction/sequence specific primer （PCR/SSP） technique. Result： The frequency of MHC-DRBI ＊ 0301 allele was significantly higher in patients than that in the controls, RR of RAS patients Was 4.696, P〈 0.05, Conclusion： These findings further Verify that immunogenetics play a predominant Pathogenetics role in a subset of RAS patients. Individuals carrying MHC-DRBI ＊ 0301 alleles may be susceptibility gene to RAS. This method is More specific, sensitive, simple, rapid and accurate and could be applied to the routine clinical detective.