高铜对雏鸡肝细胞周期和凋亡影响的研究

Effect of Dietary High Copper on the Cell Cycle and Apoptosis of Liver in Chickens

420只1日龄艾维茵肉鸡随机分为7组,分别喂以对照日粮(Cu 11 mg/kg)和高铜日粮(Cu 100 mg/kg,高铜Ⅰ组;Cu 200 mg/kg,高铜Ⅱ组;Cu 300 mg/kg,高铜Ⅲ组;Cu 400 mg/kg,高铜Ⅳ组;Cu 500 mg/kg,高铜Ⅴ组;Cu600 mg/kg,高铜Ⅵ组)6周,以流式细胞术和原位末端标记法(TUNEL)研究了高铜对雏鸡肝细胞周期和凋亡的影响。结果表明,日粮铜水平达到300 mg/kg以上时,肝细胞DNA直方图出现位于G1峰之前的亚G1峰(又叫AP峰),凋亡细胞比例与对照组比较差异显著(P<0.05)。TUNEL法检测,肝脏组织切片中出现被染成黄褐色的凋亡细胞,且检出率随日粮铜水平的升高而升高。日粮铜水平达到400 mg/kg以上时,肝细胞增殖分化受阻。

The experiment was conducted with the objective of examining the effect of dietary high copper on the cell cycle and apoptosis of liver in chickens by the methods of flow cytometry （FCM） and TUNEL. 420 one-day-old Avian chickens were randomly divided into seven groups based on diets as follows： control feed （11 mg/kg Cu） and high copper feed（100 mg/kg Cu as high copper group Ⅰ; 200 mg/kg Cu as high copper group Ⅱ; 300 mg/kg Cu as high copper group Ⅲ ; 400 mg/kg Cu as high copper group Ⅳ; 500 mg/kg Cu as high copper group Ⅴ; 600 mg/kg Cu as high copper group Ⅳ）. All groups were treated for six weeks. By FCM, the percentage of hepatocyte apoptosis was found increased in high copper groups Ⅲ, Ⅳ, Ⅴ and Ⅵ when compared with that of control group. Also, the TUNEL staining of liver tissue showed that apoptosis cells were increased in high copper groups Ⅳ, Ⅴ and Ⅵ respectively. The G0/G1 phase of cell cycle of liver was much higher, and S, G2 ＋ M phases lower in high copper groups Ⅳ, Ⅴ and Ⅵ than in control group （P〈0.05 or P〈0. 01）.