ERα免疫反应产物在山羊卵巢中的分布

Distribution of Estrogen Receptor α Immunoreactivity in Goats Ovary

为了研究不同生殖周期山羊卵巢中雌激素受体α(estrogen receptorα,ERα)的表达,本试验应用高灵敏度的免疫组织化学SP法检测了ERα免疫反应产物在不同生殖周期山羊卵巢中的分布。结果显示,ERα免疫反应产物主要存在于成熟卵泡的颗粒细胞、发情期和妊娠期生长卵泡的部分颗粒细胞、原始卵泡卵母细胞胞质、粒黄体细胞胞质及膜黄体细胞胞核、卵泡膜细胞和类固醇细胞胞质;间情期生长卵泡的颗粒细胞中的ERα免疫反应产物较少,主要存在于细胞膜上;卵母细胞中ERα免疫反应产物在初级卵泡阶段含量较少,从次级卵泡开始其含量很丰富。以上结果提示,ERα以激活不同信号途径的方式参与了对卵泡生长、排卵及妊娠维持的调控,这为研究雌激素及雌激素受体α在卵巢中的作用及其生殖调控机理提供了形态学资料。

To study the localization of estrogen receptor α （ERα） and its action in the process of reproduction during the different period of reproduction in goat ovary, supersensitive immunohistochemistry SP method was used to detect the distribution of the ERα immunoreactivity （ERα-ir） during the different period of reproduction in the goats ovary. The results showed that ERα-ir exist mainly in granulosa cells in the mature follicles, some granulosa cells in the developing follicle during oestrum and the trimester of pregnancy, cytoplasm of oocyte in primordiar follicle, cytoplasm of granular lutein cells and nucleus of theca lutein cells during the trimester of pregnancy, theca cells and cytoplasm of steroid secretory cells. The content of ERα-ir in granulosa cells in the developing follicle was few, only exist in the cytomembrane during the dioestrus. At the stage of primary follicle, the content of ERα-ir in oocyte was few. Later the content of ERα-ir was very abundant. The results suggest that ERα control the development of follicle, ovulation and pregnancy maintenance by activating different signal pathway. The above results provide morphological materials for research about the function of estrogen and estrogen receptor α in the ovary and its regulatory mechanism of reproduction.