尼膜同对大鼠脑出血后血脑屏障通透性及水通道蛋白4mRNA表达的影响

Effect of nimoton on blood-brain barrier permeability and AQP4mRNA expression after experimental cerebral hemorrhage in rats

目的研究大鼠脑出血后血脑屏障(BBB)通透性与水通道蛋白4(AQP4)的关系及尼膜同的干预作用。方法采用自体动脉血注入尾状核法制成大鼠脑出血模型,RT-PCR法观察AQP4mRNA的表达,伊文思兰法测量BBB通透性,干湿重法计算脑含水量表示脑水肿。结果与对照组相比,脑出血组及尼膜同组BBB通透性均在出血后6h开始升高(0.5955±0.0956、0.5092±0.0309),1d^3d最高(0.8889±0.0968、0.7826±0.0339和0.7914±0.0520、0.7442±0.0753),尼膜同组低于脑出血组(P<0.05);两组AQP4mRNA表达也于6h即开始升高(1.06±0.12、0.90±0.15),3d时达到高峰(1.34±0.14对1.27±0.14),尼膜同组低于脑出血组(P<0.05);BBB通透性与AQP4mRNA表达呈显著正相关(r=0.686,P<0.01),与脑水肿变化趋势一致。结论脑出血后可能通过上调APQ4mRNA表达,增加BBB通透性,参与脑水肿形成,尼膜同可抑制此过程。

Objective To investigate the relationship between BBB permeability and AQP4 and the effect of nimoton after experimental cerebral hemorrhage （ICH） in Rats. Methods The ICH models were established by stereotaxic injection of autologous artery blood into the caudate nucleus. We detected AQP4mRNA expression by RT-PCR,BBB permeability by EB method and brain water contents by dry-wet weight method. Results In contrast to the control group,BBB permeability increased at 6 hours in ICH group and nimoton group after cerebral hemorrhage, reached peak at 1～3 days,then which in nimoton group was lower than that in ICH group （P〈0.05）;AQP4mRNA expression increased at 6 hours in two groups,reached peak at 3 days,then which in nimoton group was lower than that in ICH group （P〈0. 05）. There was a significantly positive correlation between AQP4mRNA expression and BBB permeability （R = 0. 686,P〈0. 01）. They were consistent with the change of cerebral edema. Conclusion After ICH ,cerebral edema may be formed through the up-regulation of AQP4mRNA expression and the increase of BBB permeability. Nimoton can inhibit the process.