酒精戒断对清醒大鼠海马DG区部分兴奋性氨基酸的影响

Effect of ethanol withdrawal on partial excitatory amino acids levels in the hippocampus DG in conscious rats

目的探讨在清醒状态下大鼠长期饮酒停饮后出现戒断症状时海马DG(dentate gyrus)区部分兴奋性氨基酸含量变化。方法成年Wistar大鼠(300g^350g)40只随机分成对照组(n=10)和酒精组(n=30)。酒精组用50%酒精按10ml/kg/d灌胃1周(n=10)、2周(n=10)、4周(n=10),对照组给予同等剂量的生理盐水灌胃4周。用微透析法和高效液相色谱法分别测定末次饮酒6h、30h、48h、72h海马DG区谷氨酸及天冬氨酸含量,同时观察大鼠撤除酒精后不同时间戒断症状。结果2周酒精组及4周酒精组在末次饮酒30h、48h时,清醒大鼠海马DG区天冬氨酸及谷氨酸水平较末次饮酒6h显著上升(P<0.05),末次饮酒72h与末次饮酒6h无明显变化;兴奋性氨基酸含量升高时间与戒断症状明显出现时间基本一致;1周酒精组、对照组在上述各时段天冬氨酸及谷氨酸水平无显著变化。结论(1)酒精戒断症状与饮酒时间长短呈正相关;(2)酒精戒断综合征与海马DG区细胞外液兴奋性氨基酸神经递质含量增加有关。

Objective To investigate the effect of ethanol withdrawal on partial excitatory amino acid in hippocampus DG of conscious rats. Methods Forty four-month-old rats were randomized into control group（n=10） and experiment group（n= 30）. Experiment group were orally exposed to 50% ethanol（10ml/kg/d） for 1 week,2 weeks and 4 weeks and control group were exposed isovolume saline 4 weeks. The microdialysis technique and high-performance liquid chromatography was used to quantify glutamate and asptamate in hippocampus DG with each rat at 6h, 30h, 48h and 72h during the ethanol withdrawal. At the same time,the ethanol withdrawal syndromes were observed. Results （1）The glutamate and asptamate of 2-week ethanol group and 4 week ethanol group at 30h and 48h were significantly high than that of 6h during ethanol withdrawal （P〈0.05） . （2）There was no significant difference between control group and 1-week ethanol group. Conclusions （1） The ethanol withdrawal syndromes have relationship with time which was exposed to ethanol. （2） The results of this study demonstrate that excitatory amino acid levels in hippocampus DG increased with subsequent ethanol withdrawal periods.