大黄素甲醚对大鼠脑缺血预处理后缺血再灌注损伤作用的探讨

Study of the Protective Effects of Physcion on Crerbral Ischemia-reperfusion Injury in Rats after Brain Ischemic Preconditioning

目的探讨脑缺血预处理联合大黄素甲醚(Phy)治疗对大鼠脑缺血再灌注损伤的保护作用及其机制。方法SD大鼠随机分为四组,分别为假手术组、脑缺血再灌注组、脑缺血预处理组和Ply治疗组。各组采用Zea-longa等的大脑中动脉线栓法并加以改进,制备缺血预处理及缺血再灌注损伤实验动物模型。比较各组神经功能缺失评分、脑梗死体积、血清神经元烯醇化酯(NSE)含量及脑组织白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)含量。结果脑缺血预处理联合Phy可使神经功能缺损减轻、脑梗死体积缩小、血清NSE含量降低,均较缺血预处理组更为明显。脑组织IL-1β和TNF-α含量降低亦更为明显。结论Phy可增强缺血预处理诱导的脑缺血耐受作用,下调脑组织再灌注损伤时IL-1β和TNF-α的表达,二者具有叠加作用。

Objective To investigate the protective effects and the mechanisms of cerebral ischemic preconditioning combined with Physcion （Phy） on cerebral ischemia-reperfusion injury of rats. Methods SD adult rats were divided into 4 groups randomly： sham group, cerebral ischemia-reperfusion group, brain ischemic preconditioning group and Physcion treatment group.The rat model of focal cerebral ischemia was created by middle cerebral artery occlusion （MCAO） after Zea-longa by making some modification. Then comparing neurological deficits scores and the volume of cerebral infarction. The level of NSE in serum and the level of IL-1β and THF-α in cerebral tissue were also detected. Results Brain ischemic preconditioning combined with Physcion can decrease neurological deficits and reduce the volume of infarction. At the same time, the level of NSE in serum can be decreased. The effects were more significant than those only received brain ischemic preconditioning. The level of IL-1β and TNF-α in cerebral tissue also decreased much more significant. Conclusion Physcion can strengthen the effect of ischemic tolerance induced by brain ischemic preconditioning and decrease the expression of IL-1β and TNF-α, they both have additive effect.