人胎小肠NOS阳性神经元发育的免疫组化观察

An Observation on the Development of Nitric Oxide Synthase Positive Neurons in the Small Intestine of Human Fetus with Immunohistochemical Techniques

目的探讨人胎小肠一氧化氮合酶(NOS)阳性神经元生长发育规律。方法用免疫组织化学法对人胎小肠NOS阳性神经元的表达进行观察。结果从第3个月开始,随着胎龄的增加,NOS阳性神经元的细胞体逐渐增大,细胞质逐渐增多,阳性反应逐渐增强;NOS阳性神经纤维由短小突起逐渐伸长,并形成膨体纤维,在肌层分布由稀疏逐渐变为致密。结论小肠壁内NOS阳性神经元的生长发育与各层组织细胞的发育有密切关系。

Objective To explore the law of the development about NOS positive neurons in the small intestine of human fetus. Methods The expression of NOS positive neurons in the small intestine of human fetus was observed by using immunohistochemical method. Results From the third month of human fetus, with the fetal age increasing,the celt bodies of NOS positive neurons gradually enlarged, cytoplasm gradually increased and positive reaction gradually enhanced. NOS positive nerve fibers gradually developed longer from a small and short tuber, and then formed the varicosity fibers. The density of NOS positive nerve fibers gradu- ally increased in the inner circular muscle layer. Conclusion The development of NOS positive neurons is closely associated with the development of cells and tissues in the different layers of small intestine wall.