HPLC同时测定万寿菊花中叶黄素及玉米黄素的含量

Determination of lutein and zeaxanthin in Tagetes erecta L.by HPLC

目的:建立一种同时测定万寿菊花中叶黄素及玉米黄亲含量的HPLC方法。方法:采用Develosil C_(30)色谱桂(250 mm×4．6 mm,5μm),流动相为甲醇-乙腈(49:51),内含0．05％三乙胺,流速1．0 mL·min^(-1),检测波长450 nm。结果:叶黄素和玉米黄素分别在5．0～80．0和2．0～32．0μg·mL^(-1)的浓度内峰面积呈良好的线性关系(r分别为0．9998,0．9997),平均回收率分别为99．25％(RSD=2．1％,n=9)和99．41％(RSD=2．3％,n=9)。结论:本法简便准确、重现性好,可用于万寿菊花中叶黄素及玉米黄素的含量测定。

Objective： To establish a HPLC method for simultaneous quantification of lutein and zeaxanthin in Tagetes erecta L. Methods： The HPLC condition was consisted of a Develosil C30 column （250 mm ×4.6mm,5μm）, an eluate of methanol-acetonitrile （49：51 ） contaning 0.05% triethylamine with a flow rate of 1.0 mL·min^-1 and detection at 450 nm. Results ： The linearity of lutein and zeaxanthin concentrations were shown in the range of 5.0 -80.0μg·mL^-1（r =0,999 8） and 2.0 -32.0μg·mL^- （r = 0. 999 7） , respectively. The average recovery of lutein and zeaxanthin was 99.25% with RSD = 2.1% （ n = 9 ） and 99.41% with RSD = 2.3 % （ n = 9 ）, respectively. Conclusion ： The reliable HPLC method is applied to quantify lutein and zeaxanthin in Tagetes erecta L