复方气喘合剂中四种组分的毛细管区带电泳安培检测法测定

Determination of Four Components in Asthma-Mixture by Capillary Zone Electrophoresis with Ampere Detector

采用毛细管区带电泳-安培检测法测定了气喘合剂中氨茶碱、那可汀、盐酸甲氧那明和盐酸麻黄碱的含量。Cu修饰的碳纤维电极对四组分均显示出高催化活性,且稳定性好。四组分的线性范围分别为1.3～28μg/ml(r=0.997)、0.6～17μg/ml(r=0.992)、0.4～13μg/ml(r=0.996)、0.5～10.3μg/ml(r=0.995)。平均回收率分别为99.3%(RSD2.1%)、99.3%(RSD2.3%)、101.4%(RSD1.9%)、98.7%(RSD3.1%)。

A capillary zone electrophoresis method with ampere detector was established for determination of aminophylline, narcotine, methoxyphenamine hydrochloride and ephedrine hydrochloride in asthma-mixture. The copper modified microdisk carbon fiber electrode had high catalytic activity and good stability for the four components. The calibration curves were linear in the ranges of 1.3 - 28μg/ml （r=0.997）, 0.6 - 17μg/ml （r=0.992）, 0.4 - 13μg/ml （r=0.996）, and 0.5 - 10.3μg/ml （r = 0.995）, respectively. The average recoveries were 99.3 % （RSD 2.1%）, 99.3 % （RSD 2.3 % ）, 101.4 % （RSD 1.9 % ） and 98.7 % （RSD 3.1% ）, respectively.