血小板因子4体外对正常人骨髓细胞化疗药物敏感性的影响

EffectsofPF4onchemosensitivityofhumanbonemarrowcels

为证实化疗中血小板因子４（ＰＦ４）对正常骨髓细胞的保护作用，用体外培养人骨髓细胞，以ＭＴＴ法测定细胞的存活率，观察ＰＦ４预处理后，骨髓细胞对化疗药物敏感性的变化。经ＰＦ４（５μｇ／ｍｌ）预处理４８小时后，骨髓细胞对柔红霉素、阿霉素、鬼臼乙叉甙、氨甲喋呤和长春新碱的敏感性降低，药物的半数抑制浓度（ＩＣ５０）增加（Ｐ＜０．０５），其作用与ＰＦ４的浓度及预处理的时间有关。结果提示，ＰＦ４能减轻化疗药物对正常人骨髓细胞的细胞毒性。

Objective:Toverifytheprotectiveefectofplateletfactor4(PF4)onnormalbonemar-rowcels.Method:AfterpreincubationofhumanbonemarrowcelswithPF4(5μg/ml)for48hoursinliquidculture,adrugwasaddedinaserialdilutionmannertogiveafinalvolumeof200μl/wel.Controlwelscontainedmediumwithoutdrug.ThecelsurvivalratesweremeasuredbyMTTassay.50%in-hibitoryconcentration(IC50)foraparticulardrugwasdefinedastheconcentrationproducing50%de-creaseincelgrowth.Result:PreincubationwithPF4causedanincreaseintheviabilityofcelsaftertreat-mentwithindividualdrugsinvariousconcentrationsandtheIC50ofeachdrugwasalsoincreased.Theef-fectswererelatedtothepreincubationtimeandtheconcentrationofPF4.Conclusion:PF4canprotecthumanbonemarrowcelsfromthecytotoxicityofchemotherapeuticdrugs,andapossibleuseofPF4asaprotectivedrugforthetreatmentofcancerwassuggested.