摘要
目的:研究扶正消方药液对人胃癌细胞SGC-7901细胞凋亡的诱导作用,探讨扶正消方药液治疗胃癌的可能作用机理。方法:MTT法检测扶正消方药液对SGC-7091细胞增殖的影响;应用电镜和流式细胞仪检测细胞凋亡情况。结果:扶正消方药液能抑制胃癌细胞SGC-7901的增殖。在实验浓度范围内,随着剂量的增加,抑制作用更加明显;同一剂量下,72小时内药物作用时间越长,抑制作用越明显。流式细胞仪检测在细胞周期G1期前出现低于2倍体的凋亡峰,电镜检测到胃癌细胞SGC-7901发生典型的凋亡形态学改变。结论:扶正消方药液能够抑制人胃癌细胞SGC-7901的体外生长,其作用机制可能与抑制人胃癌细胞SGC-7901的增殖和诱导细胞凋亡有关。
Objective: To investigate the apoptosis of SGC-7901 cell induced by fuzhengxiaozheng prescription, study its antitumor mechanism in vitro. Methods: The proliferation of SGC-7901 cells was measured by MTT colorimetric assay, the apoptosis cell was analysised by flow cytometry. Results: Fuzhengxiaozheng prescription could inhibit the proliferation of SGC-7901 cells in dose-dependent manner and in time-dependent manner from 0h to 72h. The cells cultured with fuzhengxiaozheng prescription showed an apoptotic peak which was lower than that of diploid before G1 phase. The typical apoptosis morphology and hypodiploid changes of SGC-7901 were examined by electron microscopy. Conclusion: Fuzhengxiaozheng prescription can inhibit the growth of SGC-7901 in vitro,inhibiting its proliferation and inducing its apoptosis maybe one of its antitumor mechanism. KMBWM
出处
《中国中医药科技》
CAS
2006年第5期296-298,共3页
Chinese Journal of Traditional Medical Science and Technology
基金
浙江省中医管理局课题No.2000C85
