摘要
采用苯酚一硫酸分光光度法对杏鲍菇中多糖含量进行了测定。测定波长485啪，多糖换算因子f=1．57，在5．00-70．00μg/mL范围内吸光度与被测物含量呈良好的线性关系，相关系数r=0．9998，方法的回收率在98．6％-102．3％之间，相对标准偏差RSD在2．01％-2．94％之间。测定结果表明，杏鲍菇中含有丰富的多糖。陔法简便、准确、重现性好，可作为杏鲍菇多糖含量的测定方法。
The content of polysaccharide in Pleurotus eryngli was determined by phenol-sulfuric acid method. The wavelength for tetennination was 485 nm and converting factor of polysaccharide was 1.57. The good hnearity rang from 5.00 - 70.00 μg/mL （ r = 0.999 8）, recovery of the method were between 98.6% - 102.3%, the relative standard deviation of the method were between 2.01% - 2.94%. The results show there is higher content of polysaccharide in Pleurotus eryngii. The method is accurate. It can be used for determination of polysaccharide in Pleurotus eryngii.
出处
《广东微量元素科学》
CAS
2006年第7期53-56,共4页
Trace Elements Science
关键词
杏鲍菇
多糖
苯酚-硫酸分光光度法
提取
Pleurotus eryngii
polysaccharide
phenol-sulfuic acid method
extraction
