华山松成熟胚的器官发生研究初探

Organ culture of mature embryos in Pinus armandii Franch

探索华山松的离体培养技术,对发展该树种的遗传调控技术和建立相关快繁体系等都具有一定的理论和实际意义.该试验以华山松的成熟胚为外植体,研究了培养基、生长调节剂和蔗糖等因子对其再生体系建立各阶段的影响.结果表明:①不定芽诱导培养基为DCR+BA3.0～5.0mgL+蔗糖30gL+琼脂5.0gL,最高诱导率可达到67.57%;②在相同浓度下,BA诱导不定芽的效果好于KT;③继代培养时必须降低培养基中的无机盐和生长调节剂含量;④添加0.1mgLIBA和2gL活性炭有利于丛生芽和茎段的伸长生长;⑤在附加4.0～5.0mgLIBA的12DCR培养基上,从诱导的愈伤组织内可以分化出较粗短的不定根,但植株移栽时较难成活.试验反映出的不同树种的离体胚在培养基上放置取向效应的差异机制问题和华山松的生根问题,有待于进一步研究探讨.

There is great theoretical and practical significance in developing genetic control and establishing a revelant rapid propagation technique by exploring culture in vitro of Pinus armandii Franch. The paper studied the effecfs of media hormones and sucrose on plantlet regeneration through embryos as explants. Results showed that： 1 ） the adventitious bud induction medium： DCR ＋ 3.0-5.0 mg/L BA ＋ 30 g/L sucrose ＋ 5.0 g/ L agar, and the highest induction frequency reached 67.57%; 2）BA was more efficient than KT in the adventitious bud formation at the same concentration ; 3 ） the content of nutriments and hormones in media should be reduced when subcuhured; 4 ）an addition of 0.1 mg/L IBA and 2 g/L AC promoted the multiplication and elongation of fascicular buds and stems; 5）the stubbed adventitious roots could be induced from the calli of 1/2 DCR medium supplemented with 4.0-5.0 mg/L IBA, but the plantlets had difficulty surviving the transplantation. The problems caused by variation in the mechanism of in vitro embryos of conifers, as well as those of rooting induction, revealed in the experiment, need to be discussed further.