超临界CO_2萃取油松针叶萜烯化合物的方法研究

Supercritical carbon dioxide extraction of terpenoids from needles of Pinus tabulaeformis

为探索研究油松的新方法,该文应用超临界CO2萃取技术,以油松针叶为材料萃取萜烯类化合物,以α蒎烯、β石竹烯为研究的重点,采用单因子变化的实验方法,通过GC和GCMS监测,改变萃取时间、萃取压力、萃取温度,找到最佳实验条件.实验结果表明,萃取压力15MPa、萃取温度40℃和萃取时间20min,是从油松针叶中萃取以α蒎烯和β石竹烯为代表的萜烯类化合物得率较高的萃取条件.与水蒸气蒸馏相比,该实验方法省时且样品收率高.

Supercritical carbon dioxide extraction is an advanced technique in extracting volatile substances, which works at low temperatures, without any oxidation and deterioration. This method was first used to extract volatile terpenoids, such as α-pinene and β-caryophyllene, from the needles of Pinus tabulaeformis by means of alternating the factors of extraction pressure, temperature and time, and by GC and GC/MS monitoring. Results show that the optimum extraction conditions including the pressure of 15 MPa, temperature of 40℃ and the time of 20 min. Compared with the former method of vapour distillation, the duration of this method is shorter and extraction efficiency is higher.