减毒活卡介苗皮内注射对哮喘儿童细胞因子的影响

The Effects of Attenuated BCG Vaccine on Cytokines in Children with Asthma

目的:探讨减毒活卡介苗对哮喘患儿细胞因子的调节作用。方法:用抽签法将40例急性发作期哮喘患儿随机分为两组,每组20例。减毒活卡介苗组予全球哮喘防治创议(GINA方案)+减毒活卡介苗0.1ml皮内注射;常规治疗组单纯GINA方案治疗。酶联法检测治疗前后血清IgE及外周血单个核细胞(PBMC)诱生的白细胞介素2(IL-2)、白细胞介素12(IL-12)及γ-干扰素(IFN-γ)。结果:两组治疗后IL-2、IL-12、IFN-γ均高于治疗前(P<0.01),IgE均低于治疗前(P<0.01)。两组间治疗前后IL-2、IL-12、IFN-γ、IgE变化的幅度无显著性差异(P>0.05)。结论:5×104CFU的减毒活卡介苗皮内注射对3岁以上哮喘患儿IL-2、IL-12、IFN-γ、IgE失衡的调节无明显意义。

Objective：To study the effects of attenuated BCG vaccine on cytokines in children with asthma. Methods：40 patients with acute asthma were divided into two groups： control or intervention groups, all patients were managed according to Global Initiative for Asthma guidelines, patients in intervention group （ n = 20） were also vaccinated subcutaneously with attenuated BCG vaccine. The levels of IL-2, IL-12, IFN-γ derived from peripheral, blood mononuclear cells （PBMC） and serum IgE were measured by ELISA before and after treatment. Results：After treatment, the levels of IL-2, IL-12, IFN-γ were increased （P 〈 0. 01 ） in two groups, and the levels of IgE were decreased in two groups（ P 〈 0.01 ）. There were no significant differences in the levels of IL-2 , IL-12, IFN-γ, IgE between control and intervention groups （ P 〉 0.05 ）. Conclusion ： There were no significant effects of attenuated BCG vaccine （5 × 10^4 CFU ） on the elevated levels of IL-2,IL-12, IFN-γ, IgE in 3 - 12 years old patients with asthma.