期刊文献+

重组犬IL-2在原核细胞中的高效表达 被引量:2

High expression of canine recombinant IL-2 gene in Escherichia coli
下载PDF
导出
摘要 应用RT-PCR技术克隆犬IL-2基因,并插入到原核表达载体P JLA 605中,构建pRL-C aIL-2表达质粒;采用M 9培养基摇瓶发酵,确定诱导时机和诱导表达时间。结果表明:工程菌pRL-C aIL-2在30℃培养至对数生长后期(OD600为1.5)42℃诱导4 h时,菌体收得量湿重达17.8 g/L,目标蛋白表达量约占菌体总蛋白的29.7%。外源基因在该基因工程菌中得到了高效表达。 The canine IL-2 gene of was cloned by RT-PCR and was inserted into PJLA605 vector. The recombinant plasmid of pRL-CaIL 2 was constructed. The cell growth phases for induction and inducing period were determined using shaking flask with M9 medium. The results showed that when induced at 42 C for 4 hours after culture the E. coli DH5α(pRL-CaIFN -γ) in shaking flask reached to 1.5(OD600) at 30 C, 17.8 g of wet bacteria per liter could be obtained and the derivative of CaIL-2 was about 29. 7% of total protein in the host. The gene was highly expressed in the engineering bacterial strain.
出处 《中国兽医杂志》 CAS 北大核心 2006年第8期10-12,共3页 Chinese Journal of Veterinary Medicine
关键词 犬IL-2 高效表达 大肠杆菌 Canine IL-2 highly expression Escherichia coli
  • 相关文献

参考文献6

  • 1Taniguchi T,Matsui H,Fujia T,et al. Structure and expression of a clonedcDNA for human interleukin-2[J]. Nature,1983, 302(5906):305-310.
  • 2Collins R A,Tayton H K,Gelder K I,et al. Cloning and expression of bovine and porcine interleukin-2 in baculovirus andanalysis and species cross-reactivity [J ]. Vet Immunol Immunopathol, 1988,40 : 313-324.
  • 3Knapp D W, Williams J S,Andrisani O M. Cloning of the canine interleukin-2 encoding cDNA[J]. Gene,1995,159: 281- 282.
  • 4Goodall J C,Emery D C,Ptrry A C F,et al. cDNA cloning ofporcine interleukin-2 by polymerase chain reaction[J]. Bioch Biophys Acta, 1991,1089:257-288.
  • 5Sundick R S, Gill-Dixon C. A cloned chicken lymphokine homologous to both mammalian IL-2 and IL-15[J]. J Immunol, 1997,159(2); 720- 725.
  • 6张振龙,许丽峰,张琰平,刘德郁,邓宛明,樊晓翔,陈梅娣,窦金福,宋比天,倪道明,李昌本.pRL-hTNF/JM103工程菌发酵和rhTNFα表达的研究[J].中国生物制品学杂志,1998,11(1):14-17. 被引量:2

二级参考文献1

  • 1(美)J.萨姆布鲁克(J.Sambrook)等著,金冬雁等.分子克隆实验指南[M]科学出版社,1992.

共引文献1

同被引文献10

  • 1魏晓锋,李春华,李祥瑞,徐立新.犬白细胞介素-2基因(IL-2)的克隆与表达[J].农业生物技术学报,2004,12(3):268-272. 被引量:5
  • 2许健,樊拥军,李龙,万旺军,李建荣,于涟.鸡白细胞介素2在毕赤酵母细胞中的表达[J].西安交通大学学报(医学版),2004,25(4):339-342. 被引量:11
  • 3MERLUZZI V J,WELTE K,SAVAGE D M,et al. Expansion of cyclophosphamide-resistant cytotoxic precursors in vitro and in vivo by purified human interleukin-2[ J]. J Immunol,1983,131:806 -809.
  • 4HAZAMA M,MAGUMI A A,ASAKSW N,et al. Adjuvant-independent enhanced immune responses torecombinant herpes simplex virus type 1 glycoprotein D by fusion with biologically active interleukin - 2 [ J ]. Vaccine, 1993,11:629 -636.
  • 5LI J R,LIANG X Y,HUANG Y W,et al. Enhancement of the immunogenicity of DNA vaccine against infectious bursal disease vires by co - delivery with plasmid encoding chicken interleukin 2[J]. Virology,2004,329:89 - 1130.
  • 6KASHIMA N ,NISHI T. CUnique structure of murine interleukin-2 as deduced from clonedcDNAs [ J ]. Nature, 1985,313:402 - 404.
  • 7CERRET17 D P,MCKEREGHAN K,LARSEN A,et al. Cloning,sequence, and expression of bovine interleukin-2[ J] . Proc Natl Acad Sci USA,1986, 83:3223 - 3227.
  • 8TANIGUCHI T,MATSUI H,FUJITA T,et al. Structure and expression of a cloned cDNA for human interlukin-2[ J]. Nature,1983,302:305 - 310.
  • 9MCHNIGHT A J ,MASON D W,BARCLAY A N. Sequence of.rat interleu- kin 2 and anomalous binding of a mouse interleukin 2 cDNA probe to rat MHC class II - associated invariant chain mRNA [ J ]. Immunogenetics, 1989.30.145 - 147.
  • 10费聿锋,钱建飞,凌雯,李银,张则斌,王贵升,李祥瑞.重组鸡IL-2增强鸡四联灭活苗免疫效果的研究[J].畜牧与兽医,2004,36(4):9-11. 被引量:18

引证文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部