摘要
通过RT-PCR方法从大鼠骨骼肌中克隆到肌肉素cDNA,构建表达载体pGEX-5X-3-musclin,并在BL21大肠杆菌中成功表达了融合蛋白GST-Musclin,且对表达条件进行了优化.在最优化的表达条件下,融合蛋白的表达量达到了14.2%.
In this paper, musclin cDNA was cloned from skeletal muscle of rat by RT-PCR. Then the gene fragment was cloned into expression vector pGEX-Sx-3, and the recombinant was transformed into E coli BL21. Induced by IPTG, the fusion protein GST-musclin, a 40 kDa protein was expressed in E coli BL21. and identified in SDS-PAGE In the optimum conditions, the expression level of fusion protein was 14.2%.
出处
《江西师范大学学报（自然科学版）》
CAS
北大核心
2006年第4期387-391,共5页
Journal of Jiangxi Normal University(Natural Science Edition)
关键词
肌肉素
克隆
表达
musclin
cloning
expression
