人成骨细胞与异体脱钙松质骨成骨作用的实验研究

Osteogenic action of human osteoblasts and alloplastic decalcified cancellous bone

目的观察人成骨细胞体外培养和接种于异体脱钙松质骨的成骨过程及特点。方法采用正常人骨膜分离提取成骨细胞在体外进行培养,扩增,观察成骨细胞形态和生长情况,然后将其接种于异体脱钙松质骨支架上,植入裸鼠体内进行培养,8周后对大体标本进行扫描电镜的组织学检查,观察成骨情况。结果人骨膜分离、培养、扩增的成骨细胞在体外生长良好,增殖旺盛。成骨细胞接种于异体脱钙松质骨植入裸鼠体内培养。8周后组织学观察支架有块状断层吸收,有岛状新生成骨。结论成骨细胞接种于异体脱钙骨松质骨能钙化成骨,为骨缺损的修复提供骨源成为可能。

Objective To study osteogenic courses and features of human osteoblasts cultured in vitro and seeded into alloplastic decalcified cancellous bone. Methods Human osteoblasts isolated from normal human periosteum were cultured and amplified in vitro. Thy configuration and developing manners of osteoblasts were observed. Then osteoblasts were seeded into alloplastic decalcified cancellous bone. Thy composites were implanted and cultured in thy body of athymic mice. The specimens were obtained 8 weeks later. Osteogenic characteristics were observed by scanning electron microscope. Results Osteoblasts isolated from human periosteum developed well and proliferated rapidly in vitro. Island new bone formation could be observed histologically 8 weeks after the composites of osteoblasts and alloplastic decalcified cancellous bone were implanted and cultured in the body of athymic mice. Conclusion Calcified bone can be formed by seeding osteoblasts into alloplastic decalcified cancellous bone,which may be a new kind of bone graft source to construct bone defects.