青春期单纯性肥胖雄性大鼠模型的实验研究

Experimental Study of Diet-induced Obesity Model in Pubertal Male Rats

目的：以高脂饲料配方建立青春期单纯性肥胖雄性大鼠模型。方法：21d龄雄性清洁级SD大鼠随机分为对照组（n=28只，喂普通饲料）和实验组（n=45只，喂高脂饲料）。观察比较3-6周末（即鼠龄函9周）大鼠的体质量、Lee＇s指数、体长和附睾周围脂肪重量。结果：喂养后第3-6周末实验组体质量、Lee’s指数和附睾周围脂肪重量均高于对照组（P〈0．01）；第3周末实验组体质量已明显超过对照组（超重23．0％），随着大鼠周龄的增加，肥胖程度也在逐渐加重，至第6周末时体质量进一步超过对照组（超重26．6％）；实验组肥胖鼠29只，非肥胖鼠16只。结论：以高脂饲料喂养的青春期大鼠可致单纯性肥胖。

Objective： To explore the establishment of diet-induced obesity model in pubertal male rats with high fat diet. Methods： Twenty-one-day-old male SD rats were randomly divided into the control group（ n = 28）with common diet and the experimental group（ n = 45）with high fat diet. Then the body weight, Lee＇s index and body length were measured after feeding 3-6 weeks （the rats age were 6-9 weeks old at last）. Results： Compared with the control group, the body weight, Lee＇s index and the fat of epididymis in experimental group increased obviously（ P 〈 0.01）. The body weight in experimental group was higher 23 % than that in control group at 6 weeks old. With the development of obesity, the body weight of obese rats was over 26.6 % more than that of the control group in the end of experiments. In experimental group, there were 29 obese rats and 16 no obese rats. Conclusion： The high fat diet can induce obesity in pubertal rats.