肝区微波照射后肝脏HSP70的表达及机制初探

Expression of Hepatic Heat Shock Protein 70 Induced by Microwave Irradiation on Liver Territory in Rabbits

目的 采用20W、2450MHz微波照射家免肝区，观察照射过程中肝前区温度及肛温的变化，并于24小时后从家兔肝右中方叶前缘取组织用免疫组化方法检测HSP70的表达。方法 健康成年家免13只，对其中4只在微波照射过程中肝前区温度及肛温的变化进行测定，其余9只分为两组，一组（n=4）取肝组织用免疫组化方法观察照射前肝HSP70的表达，另一组（n=5）进行微波预处理，24小时后观察肝脏HSP70的表达。结果 肝区微波照射过程中，家兔肝前区温度升高0．5～2．2℃，肛温升高0．5～0．7℃，24小时后肝细胞核内有HSP70的高表达，而预处理前动物肝细胞内无或仅有低水平HSP70的表达。结论 肝区微波照射过程中肝前区温度及肛温有轻度升高，24小时后肝细胞核内有HSP70的高表达。

Objetive To observe the change of intra - anual and peri - hepatic temperature during the period of microwave preconditioning, the expression of HSP70 in hepatocytes was also examined with immunohistochemical method 24h after pretreatment. Method The body and peri - hepatic temperature of 4 of 13 rabbits（group 1, n = 4） were measured with thermometers placed in anus and between the abdominal peritoneal cavity and the liver surface, respectively. The other l0 rabbits were divided randomly into 2 groups. The specimen were collected from the right middle lobe of the liver of the rabbits in group 2（n = 4） and group 3（n = 5） to dectet the expression level of HSP70 in hepatocytes before and after microwave preconditioning, respectively. Results The intra - anal and perihepatic temperture rasied 0.5 - 0.7 ℃ and 0.5 - 2.2 ℃ during the period of microwave preconditioning respectively, and HSP70 was highly expressed in hepatocytes pretreated 24 h later. Conclusion There was a mild rise in temperature of intra - anus and peri - liver during the preconditioning and HSP 70 was highly expressed in the hepatocytes 24 h after proconditioning.