摘要
建立高效液相色谱法测定复方茶碱麻黄碱片中可可碱、茶碱、盐酸麻黄碱及咖啡因的含量测定方法。采用Kromasil C18色谱柱；流动相：0．05mol·L^-1磷酸二氢钾溶液-甲醇-三乙胺（72：28：0．2）；检测波长：215nm；流速：1．0ml·min^-1；柱温：42℃。线性范围：可可碱为5～125μg·ml^-1（r=0．9999，n=6），茶碱为5～125μg·ml^-1（r=0．9999，n=6），盐酸麻黄碱2～50μg·ml^-1（r=0．9996，n=6），咖啡因3～75μg·ml^-1（r=0．9999，n=6）；平均回收率（X±SD）分别为：可可碱（99．9±0．19）Y0，茶碱（99．9±0．26）％，盐酸麻黄碱（99．6±0．33）％，咖啡因（99．8±0．23）％。本方法简便、准确、重现性好，适用于复方茶碱麻黄碱片的质量控制。
A HPLC method for the determination of theobromine, theophylline, ephedrine hydrochloride and caffeine was established. A Kromasil C18 column was used with 0.05mol · L^-1 potassium dihydrogen phosphate- methanol - triethylamine （72 ： 28 ： 0. 2） as mobile phase. The flow rate was 1. 0ml · min^-1 and column temperature was 42℃. The detecting wavelength was 215nm. The liner range was 5～125 μg·ml^-1 （r=0. 9999, n=6）, 5～125 μg·ml^-1 （r=0. 9999, n=6）, 2～50μg·ml^-1 （r =0.9996, n=6） and 3～75μg·ml^-1 （r=0.9999, n=6） for theobromine, theophylline, ephedrine hydrochloride and caffeine respectively. The average recovery rate （X±SD）% was 99.9±0. 19, 99. 9±0.26, 99.6 ± 0. 33, and 99. 8 ± 0.23 respectively. The method was simple, accurate and repeatable. It could be used in the quality control of compound Chajian Tablets.
出处
《中国药事》
CAS
2006年第9期541-543,共3页
Chinese Pharmaceutical Affairs
