摘要
建立高效液相色谱法测定复方茶碱麻黄碱片中可可碱、茶碱、盐酸麻黄碱及咖啡因的含量测定方法。采用Kromasil C18色谱柱;流动相:0.05mol·L^-1磷酸二氢钾溶液-甲醇-三乙胺(72:28:0.2);检测波长:215nm;流速:1.0ml·min^-1;柱温:42℃。线性范围:可可碱为5~125μg·ml^-1(r=0.9999,n=6),茶碱为5~125μg·ml^-1(r=0.9999,n=6),盐酸麻黄碱2~50μg·ml^-1(r=0.9996,n=6),咖啡因3~75μg·ml^-1(r=0.9999,n=6);平均回收率(X±SD)分别为:可可碱(99.9±0.19)Y0,茶碱(99.9±0.26)%,盐酸麻黄碱(99.6±0.33)%,咖啡因(99.8±0.23)%。本方法简便、准确、重现性好,适用于复方茶碱麻黄碱片的质量控制。
A HPLC method for the determination of theobromine, theophylline, ephedrine hydrochloride and caffeine was established. A Kromasil C18 column was used with 0.05mol · L^-1 potassium dihydrogen phosphate- methanol - triethylamine (72 : 28 : 0. 2) as mobile phase. The flow rate was 1. 0ml · min^-1 and column temperature was 42℃. The detecting wavelength was 215nm. The liner range was 5~125 μg·ml^-1 (r=0. 9999, n=6), 5~125 μg·ml^-1 (r=0. 9999, n=6), 2~50μg·ml^-1 (r =0.9996, n=6) and 3~75μg·ml^-1 (r=0.9999, n=6) for theobromine, theophylline, ephedrine hydrochloride and caffeine respectively. The average recovery rate (X±SD)% was 99.9±0. 19, 99. 9±0.26, 99.6 ± 0. 33, and 99. 8 ± 0.23 respectively. The method was simple, accurate and repeatable. It could be used in the quality control of compound Chajian Tablets.
出处
《中国药事》
CAS
2006年第9期541-543,共3页
Chinese Pharmaceutical Affairs